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Published on: October 31, 2014
Studies on Clostridium acetobutylicum ginA promoters and antisense RNA
P J Janssen1, D T Jones1, D R Woods1
1Department of Microbiology, University of Cape Town. Rondebosch, 7700 South Africa.
Abstract:
The Clostridium acetobutylicum glnA gene has two transcript start sites under the control of promoters P1 and p2 . Initiation of transcription was regulated by nitrogen and a downstream region was implicated in the regulation of transcript initiation by nitrogen in Escherichia coli. Putative antisense RNA was produced from a single downstream transcript start site under the control of P3. An up-promoter mutation in P3 resulted in lower levels of glutamine synthetase (GS) activity. Putative antisense RNA had a role in down-regulating GS expression but was not involved in regulation by nitrogen. Deletion of downstream inverted repeat sequences resulted in very low levels of GS activity.
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