Related Experiment Video
Updated: Feb 25, 2026
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
Published on: January 12, 2020
Microarray expression profiling of long non-coding RNAs in epithelial ovarian cancer
Ye Ding1, Da-Zheng Yang1, Yong-Ning Zhai1
1State Key Laboratory of Reproductive Medicine, Department of Endoscopic Diagnostic and Treatment Center, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
This study identified numerous differentially expressed long non-coding RNAs (lncRNAs) in epithelial ovarian cancer, revealing their potential roles in tumor development and progression. These lncRNAs may serve as novel biomarkers for ovarian cancer research.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in human cancers.
- The specific regulatory functions of lncRNAs in epithelial ovarian cancer development and progression are not fully understood.
Purpose of the Study:
- To investigate differentially expressed lncRNAs in epithelial ovarian cancer.
- To explore the potential functions of these dysregulated lncRNAs.
Main Methods:
- Microarray analysis of lncRNA profiles in ovarian cancer tissues versus adjacent normal tissues.
- Validation of selected lncRNAs using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
- Functional analysis using Gene Ontology (GO) and pathway analysis.
Main Results:
- Identified 672 upregulated and 549 downregulated lncRNAs in epithelial ovarian cancer tissues.
- Validated expression changes for specific lncRNAs, including RP11-1C1.7, XLOC_003286, growth arrest-specific 5, ZNF295-AS1, protein tyrosine kinase 7, maternally expressed gene 3, AC079776.2, and ribosomal protein lateral stalk subunit P0 pseudogene 2.
- Functional analysis suggested significant alterations in candidate genes associated with differentially expressed lncRNAs.
Conclusions:
- Significant differences in lncRNA expression profiles exist between epithelial ovarian cancer cells and normal cells.
- These dysregulated lncRNAs offer potential insights into ovarian cancer pathogenesis.
- Identified lncRNAs may serve as novel molecular biomarkers for epithelial ovarian cancer.
