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Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable

S Barreda-García1, R Miranda-Castro, N de-Los-Santos-Álvarez

  • 1Departamento de Química Física y Analítica, Universidad de Oviedo, Julián Clavería 8, Oviedo, Spain. mjlc@uniovi.es.

Chemical Communications (Cambridge, England)
|August 8, 2017
PubMed

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...