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Related Experiment Video

Updated: Feb 25, 2026

Transvaginal Mesh Insertion in the Ovine Model
10:32

Transvaginal Mesh Insertion in the Ovine Model

Published on: July 27, 2017

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Transvaginal Mesh Insertion in the Ovine Model.

Iva Urbankova1, Geertje Callewaert2, Nikhil Sindhwani3

  • 1Centrum for Surgical Technologies, Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven; Institute for the Care of Mother and Child and Third Faculty of Medicine, Charles University, Prague; iva.urbankova@upmd.eu.

Journal of Visualized Experiments : Jove
|August 9, 2017
PubMed
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This study details a sheep model for testing surgical mesh implants in the rectovaginal septum. The technique, using a single vaginal incision, mimics pelvic organ prolapse surgery in humans.

Area of Science:

  • Surgical Innovation
  • Animal Models
  • Pelvic Floor Reconstruction

Background:

  • Pelvic organ prolapse (POP) affects many women, necessitating effective surgical interventions.
  • Current surgical techniques for POP often involve mesh implantation.
  • Large animal models are crucial for pre-clinical evaluation of novel surgical devices.

Purpose of the Study:

  • To describe a novel surgical protocol for mesh insertion into the rectovaginal septum in sheep.
  • To evaluate a technique for deploying anchoring arms using a trocar-guided, "outside-in" approach.
  • To provide a reproducible large animal model for testing new pelvic reconstructive implants.

Main Methods:

  • A single vaginal incision was used for dissection and mesh/implant insertion in parous sheep.

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  • Anchoring arms were inserted using a trocar-guided technique, with cranial arms passing through specific pelvic muscles.
  • Anatomical landmarks were identified, and potential risks like peritoneal penetration were noted.

    • Main Results:

    • The described technique allows for the dissection and implantation of mesh with or without anchoring arms in the ovine rectovaginal septum.
    • Identification of anatomical spaces and critical structures was feasible.
    • Potential complications, such as trocar penetration, were identified and mitigation strategies discussed.

    Conclusions:

    • This sheep model provides a viable platform for experimental testing of novel surgical implants for pelvic organ prolapse.
    • The described trocar-guided anchoring arm insertion technique, while effective in the model, requires careful execution to avoid complications.
    • Further research may refine this technique for pre-clinical assessment of innovative pelvic reconstructive devices.