Saccharopolyspora hattusasensis sp. nov., isolated from soil

A Saccharopolyspora strain, designated CR3506^T, isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395^T (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798^T (98.4%) and Saccharopolyspora shandongensis 88^T (98.1%); low levels of DNA-DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (<50%). Strain CR3506^T was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (>10%) were MK-9(H₄) and MK-8(H₄). Major fatty acids were (>10%) iso-C_16:0, C_15:03OH, C_18:0 and iso-C_15:0. Further, the morphological, physiological and biochemical characteristics of strain CR3506^T are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0-98.5%). Strain CR3506^T has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506^T represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506^T (=KCTC 29104^T = DSM 45715^T).