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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
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Time-resolved spectroscopy at 19,000 lines per second using a CMOS SPAD line array enables advanced biophotonics

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    This study introduces the fastest time-correlated single photon counting (TCSPC) system for time-resolved fluorescence spectra (TRFS) acquisition. The novel SPAD-based line sensor enables rapid, time-gated spectral analysis for diverse applications.

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    Area of Science:

    • Photonics and Spectroscopy
    • Biomedical Optics
    • Materials Science

    Background:

    • Time-resolved fluorescence spectra (TRFS) are crucial for analyzing dynamic molecular processes.
    • Existing TCSPC systems face limitations in acquisition speed and flexibility.
    • Advancements in SPAD sensor technology offer potential for faster and more versatile spectral measurements.

    Purpose of the Study:

    • To develop and present a novel SPAD-based line sensor for ultra-fast TRFS acquisition.
    • To enhance existing TCSPC capabilities with parallelized interfaces and digital time-gating.
    • To demonstrate the sensor's performance across various spectroscopic applications.

    Main Methods:

    • Fabrication of a SPAD-based line sensor using 130 nm CMOS technology.
    • Implementation of a parallelized interface for high line rate data transfer (19,000 lines/s).
    • Development of a novel digital time-gating architecture rejecting photons in the OFF region.
    • On-chip center-of-mass (CMM) calculations for direct lifetime estimation.

    Main Results:

    • Achieved the fastest reported TRFS acquisition to date at 8.3 milliseconds.
    • Demonstrated high line rate capability enabling rapid data acquisition.
    • Successfully applied the sensor to time-gated TRFS of chlorophyll A, FRET probes, and lung tissue autofluorescence.
    • Showcased time-gated Raman spectroscopy of toluene.
    • Achieved spectrally resolved detection of fast chlorophyll A fluorescence lifetime transients.

    Conclusions:

    • The developed SPAD-based line sensor represents a significant advancement in TRFS acquisition speed and versatility.
    • The novel time-gating and on-chip CMM capabilities offer powerful tools for researchers.
    • The system's performance empowers widespread applications in spectroscopy, biosensing, and biomedical imaging.