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Related Experiment Video

Updated: Feb 25, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Compact lensless off-axis transmission digital holographic microscope.

Manon Rostykus, Christophe Moser

    Optics Express
    |August 10, 2017
    PubMed
    Summary

    This study introduces a simple, low-cost lensless holographic microscope using an off-axis design. This innovation enables faster phase imaging of cells, making advanced microscopy accessible for education.

    Area of Science:

    • Microscopy and Imaging
    • Optical Physics
    • Biomedical Engineering

    Background:

    • Existing compact lensless holographic microscopes often require complex algorithms for phase image retrieval, limiting real-time visualization.
    • Current methods rely on multiple angles or wavelengths, increasing system complexity and computational load.

    Purpose of the Study:

    • To develop a simplified, compact, lensless transmission holographic microscope.
    • To enable faster, potentially real-time, phase imaging of biological samples.
    • To create an affordable microscopy solution for educational purposes.

    Main Methods:

    • An off-axis holographic configuration was employed to simplify computational processing.
    • Side illumination and analog hologram gratings were used to shape reference and signal beams from a single light source.

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  • The system was experimentally validated using cell imaging.

    • Main Results:

    • The proposed off-axis lensless holographic microscope significantly reduces computational complexity for phase image visualization.
    • Experimental results demonstrate successful imaging of cells with a field of view of approximately 12 mm².
    • A resolution of approximately 3.9 μm was achieved, suitable for observing cellular structures.

    Conclusions:

    • The developed lensless holographic microscope offers a simplified and cost-effective approach to phase imaging.
    • The system's design facilitates real-time imaging capabilities using smartphone processors.
    • This technology is well-suited for broad educational dissemination due to its low cost and ease of use.