Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.9K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.9K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.7K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UHPLC-MS/MS analysis of PFAS in the serum of patients with Alzheimer's disease, mild cognitive impairment and controls: A preliminary study.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2025
Same author

Quantitative structure retention relationship (QSRR) modelling for Analytes' retention prediction in LC-HRMS by applying different Machine Learning algorithms and evaluating their performance.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2022
Same author

Analysis of urinary organic acids by gas chromatography tandem mass spectrometry method for metabolic profiling applications.

Journal of chromatography. A·2021
Same author

Biodegradation of expanded polystyrene by mealworm larvae under different feeding strategies evaluated by metabolic profiling using GC-TOF-MS.

Chemosphere·2021
Same author

Population Pharmacokinetics of Teicoplanin in Preterm and Term Neonates: Is It Time for a New Dosing Regimen?

Antimicrobial agents and chemotherapy·2020
Same author

Targeted urine metabolomics in preterm neonates with intraventricular hemorrhage.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2018

Related Experiment Video

Updated: Feb 25, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.5K

Hyphenated MS-based targeted approaches in metabolomics.

O Begou1, H G Gika, I D Wilson

  • 1Department of Chemistry, Aristotle University, 54124 Thessaloniki, Greece.

The Analyst
|August 10, 2017
PubMed
Summary

Targeted metabolomics, focusing on specific metabolites, is gaining traction over untargeted approaches. This review covers recent advances in hyphenated chromatographic methods like LC-MS/MS for life and food sciences.

More Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.5K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.8K

Related Experiment Videos

Last Updated: Feb 25, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.5K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.5K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.8K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Metabolomics

Background:

  • Untargeted metabolomics has been a major research focus for decades.
  • Targeted metabolomics, a more hypothesis-driven approach, is increasingly utilized.
  • Targeted metabolomics aims for precise determination and absolute quantification of predefined metabolites.

Purpose of the Study:

  • To review recent developments in hyphenated chromatographic methods for targeted metabolomics and lipidomics.
  • To highlight applications of these methods in life sciences, food science, and plant science.
  • To discuss practical challenges and limitations associated with targeted metabolomic approaches.

Main Methods:

  • Focus on hyphenated chromatographic techniques: Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS).
  • Capillary Electrophoresis-Mass Spectrometry/Mass Spectrometry (CE-MS/MS).
  • Gas Chromatography-Mass Spectrometry/Mass Spectrometry (GC-MS/MS).

Main Results:

  • Technological advancements facilitate the analysis of numerous well-characterized metabolites in complex matrices.
  • These methods enable hypothesis-driven research with a focus on specific metabolic pathways or compounds.
  • Applications span diverse fields including biological research and food/plant analysis.

Conclusions:

  • Hyphenated chromatographic methods are crucial for advanced targeted metabolomic and lipidomic studies.
  • Continued technological development enhances the capability and scope of these analytical techniques.
  • Addressing practical challenges is essential for the successful implementation of targeted metabolomics.