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Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways
Published on: December 13, 2024
Airborne fungi in an intensive care unit
C L Gonçalves1, F V Mota2, G F Ferreira1
1Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Abstract:
The presence of airborne fungi in Intensive Care Unit (ICUs) is associated with increased nosocomial infections. The aim of this study was the isolation and identification of airborne fungi presented in an ICU from the University Hospital of Pelotas - RS, with the attempt to know the place's environmental microbiota. 40 Petri plates with Sabouraud Dextrose Agar were exposed to an environment of an ICU, where samples were collected in strategic places during morning and afternoon periods for ten days. Seven fungi genera were identified: Penicillium spp. (15.18%), genus with the higher frequency, followed by Aspergillus spp., Cladosporium spp., Fusarium spp., Paecelomyces spp., Curvularia spp., Alternaria spp., Zygomycetes and sterile mycelium. The most predominant fungi genus were Aspergillus spp. (13.92%) in the morning and Cladosporium spp. (13.92%) in the afternoon. Due to their involvement in different diseases, the identified fungi genera can be classified as potential pathogens of inpatients. These results reinforce the need of monitoring the environmental microorganisms with high frequency and efficiently in health institutions.
Insights
Airborne fungi in Intensive Care Units (ICUs) pose a risk for hospital-acquired infections. This study identified common fungal genera in an ICU, highlighting the need for environmental monitoring to protect patients.
Area of Science:
- Environmental microbiology
- Hospital infection control
- Mycology
Background:
- Airborne fungi in Intensive Care Units (ICUs) are linked to increased nosocomial infections.
- Understanding the environmental microbiota of ICUs is crucial for patient safety.
Purpose of the Study:
- To isolate and identify airborne fungi present in an ICU at the University Hospital of Pelotas - RS.
- To characterize the environmental fungal microbiota within the ICU setting.
Main Methods:
- Utilized 40 Petri plates with Sabouraud Dextrose Agar for sample collection.
- Samples were collected from strategic locations within the ICU over ten days, during both morning and afternoon periods.
- Identified fungal genera using standard microbiological techniques.
Main Results:
- Seven fungal genera were identified: Penicillium spp. (15.18%), Aspergillus spp., Cladosporium spp., Fusarium spp., Paecelomyces spp., Curvularia spp., Alternaria spp., Zygomycetes, and sterile mycelium.
- Penicillium spp. was the most frequent genus overall.
- Aspergillus spp. predominated in the morning (13.92%), while Cladosporium spp. predominated in the afternoon (13.92%).
Conclusions:
- The identified fungal genera, including Penicillium, Aspergillus, and Cladosporium, are potential pathogens capable of causing disease in hospitalized patients.
- Regular and efficient monitoring of environmental microorganisms in healthcare institutions is essential to mitigate infection risks.
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