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Development of Time-course Oxygen Binding Analysis for Hemoglobin-based Oxygen Carriers

Yukihiro Okamoto1, Shigenori Sugisaki1, Keishi Suga1

  • 1Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|August 11, 2017
PubMed

Developing blood substitutes is in urgent demand for chronic blood shortage all over the world. In this connection, the oxygen binding behavior of hemoglobin-based oxygen carriers (HBOCs) is one of the most important characteristics. However, present methods available for estimating oxygen binding behavior have need of expensive apparatus, and also are not suitable for high-throughput and the time-course analysis. To overcome these problems, we proposed a simple analysis method for the time-course oxygen binding behavior of HBOCs, which employs a general UV-Vis microplate reader and a common reagent, sodium dithionite, as a reductant for HBOCs and an oxygen scavenger. Our method enabled time-course oxygen binding behavior analysis of HBOCs in a simple manner, and obtained data corresponding with those by the conventional method. Thus, our developed method will accelerate the development of HBOCs due to easy oxygen binding analysis.

Keywords:
Hemoglobin based oxygen carrier (HBOCs)UV-Vis spectroscopyoxygen bindingsodium dithionite

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