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Sensitive Molecular Diagnostics for Cutaneous Leishmaniasis.

Orli Sagi1, Anat Berkowitz1,2, Shlomi Codish3

  • 1Clinical Parasitology.

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|August 11, 2017
PubMed
Summary
This summary is machine-generated.

A new multiplex qPCR test accurately diagnoses cutaneous leishmaniasis (CL) and identifies Leishmania species from lesion swabs. This rapid diagnostic tool aids in managing CL caused by Old World and New World Leishmania strains.

Keywords:
Leishmania majorLeishmania speciesqPCRreal-time PCR.

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Area of Science:

  • Medical Parasitology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Cutaneous leishmaniasis (CL) diagnosis and species identification are crucial for effective disease management.
  • In Israel, CL is primarily caused by Leishmania major and Leishmania tropica.
  • Accurate and rapid diagnostic methods are needed for timely treatment.

Purpose of the Study:

  • To develop and validate a point-of-care diagnostic test for Leishmania species.
  • To enable rapid and accurate identification of Leishmania parasites in clinical samples.
  • To differentiate between Old World and New World Leishmania species.

Main Methods:

  • Establishment of a lesion-swabbing technique for sample collection.
  • Development of a sensitive multiplex real-time PCR (qPCR) assay.
  • Utilized three probes for general Leishmania, L. major, and L. tropica detection.

Main Results:

  • Screened 1783 clinical samples over two years.
  • Detected Leishmania species in 1086 individuals (1008 L. major, 70 L. tropica).
  • Identified L. braziliensis and L. infantum/donovani in 8 samples using a secondary multiplex qPCR.

Conclusions:

  • The multiplex qPCR test provides accurate diagnostics for Leishmania infections.
  • Enabled subtyping of dominant Leishmania strains in the region.
  • Facilitated better treatment strategies for CL patients, including travelers.