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Published on: December 6, 2021
H2-Driven biocatalytic hydrogenation in continuous flow using enzyme-modified carbon nanotube columns
Ceren Zor1, Holly A Reeve2, Jonathan Quinson1
1Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK. kylie.vincent@chem.ox.ac.uk and Department of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, UK.
We developed an immobilized enzyme system for hydrogen-driven biocatalysis in flow. This platform enables efficient NADH recycling and selective biotransformations for hydrogenation reactions.
Area of Science:
- Biocatalysis
- Enzyme immobilization
- Flow chemistry
Background:
- Hydrogen-driven biocatalysis offers a sustainable alternative for chemical synthesis.
- Efficient cofactor regeneration, such as nicotinamide adenine dinucleotide (NADH), is crucial for economic viability.
- Enzyme immobilization is key to developing robust and reusable biocatalytic systems.
Purpose of the Study:
- To implement an immobilized enzyme system for hydrogen-driven NADH recycling.
- To couple NADH recycling with selective biotransformations for flow biocatalysis.
- To establish a versatile platform for various hydrogenation reactions.
Main Methods:
- Immobilization of enzymes for H2-driven NADH regeneration.
- Integration of NADH recycling with selective biotransformation in a flow system.
- Application of the system to enantioselective ketone reduction and reductive amination.
Main Results:
- Successful implementation of a flow system for H2-driven biocatalysis.
- Demonstration of efficient NADH recycling coupled to selective biotransformations.
- Validation of the platform for enantioselective ketone reduction and reductive amination.
Conclusions:
- The developed system provides a robust platform for H2-driven biocatalysis in flow.
- This approach is adaptable for a broad range of hydrogenation reactions.
- The system facilitates sustainable and efficient chemical synthesis using immobilized enzymes.
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