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High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
Published on: October 31, 2012
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Changes in protein serum levels during stem cell transplantation.
Elisabetta Metafuni1, Sabrina Giammarco1, Daniela Giovanna De Ritis2
1Hematology Department, Fondazione Policlinico Agostino Gemelli, Rome, Italy.
European Journal of Clinical Investigation
|August 11, 2017
Summary
Biomarker proteins like ST2 and BAFF show promise in predicting outcomes after stem cell transplants. Their levels correlate with graft-versus-host disease (GvHD) severity and patient survival.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Graft-versus-host disease (GvHD) is a significant complication following stem cell transplantation, impacting transplant-related mortality.
- Numerous serum proteins have been investigated as potential biomarkers for GvHD.
Purpose of the Study:
- To investigate the correlation between the concentration trends of five key serum proteins and outcomes after hematopoietic stem cell transplantation (HSCT).
- To evaluate the predictive value of these proteins for post-transplant survival.
Main Methods:
- Serum concentrations of REG3α, ST2, B-cell activating factor (BAFF), CXCL9, and elafin were measured in 77 HSCT patients.
- Blood samples were collected at baseline, day +30, GvHD onset, and GvHD resolution.
Main Results:
- REG3α levels correlated with acute GvHD; elafin and ST2 levels varied with both acute and chronic GvHD.
- BAFF concentration showed an inverse association with acute GvHD development.
- Baseline BAFF and ST2 levels predicted post-HSCT survival, while CXCL9 showed no association.
Conclusions:
- Serum protein levels, except for CXCL9, appear to fluctuate with GvHD development, irrespective of organ involvement or grading.
- Pretransplant ST2 and BAFF levels are significant predictors of survival post-HSCT.
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