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A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium

A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium

Published on: February 7, 2016

A novel organotypic 3D sweat gland model with physiological functionality.

Patricia Klaka¹, Sabine Grüdl¹, Bernhard Banowski¹

¹Henkel AG & Co. KGaA, Düsseldorf, Germany.

|August 11, 2017

Summary

Researchers developed a novel 3D sweat gland model using primary human cells. This organotypic model accurately mimics in vivo sweat gland function, offering a reliable system for drug screening and understanding sweat disorders.

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Area of Science:

Biotechnology
Dermatology
Physiology

Background:

Dysregulated eccrine sweat glands cause health issues like hyperhidrosis and anhidrosis.
Understanding sweat gland mechanisms and developing in vitro models are crucial for drug screening.

Purpose of the Study:

To develop and validate a novel organotypic three-dimensional (3D) sweat gland model.
To assess the in vitro-in vivo correlation of the 3D model for drug screening.

Main Methods:

Primary human eccrine sweat gland cells cultured using hanging drop technology to form a 3D model.
Transcriptomic and proteomic analysis to compare marker expression with in vivo human sweat glands.
Calcium flux assays to evaluate physiological responses to cholinergic stimulation.

Main Results:

The 3D model successfully regained physiological markers lost in 2D cultures.
High in vitro-in vivo correlation demonstrated through marker profiling.
Key regulators like CEACAM5, CHRM3, NKCC1, ANO1, and AQP5 were significantly expressed.
The 3D model showed a physiological response to cholinergic stimulation, unlike the NCL-SG3 cell line.

Conclusions:

The organotypic 3D sweat gland model offers superior physiological relevance compared to 2D cultures.
This model demonstrates a strong in vitro-in vivo correlation, making it suitable for drug discovery.
It serves as a valuable alternative for screening bioactives that regulate sweat mechanisms.