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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Published on: October 17, 2022

Acoustofluidic bacteria separation.

Sixing Li¹, Fen Ma², Hunter Bachman³

¹The Molecular, Cellular and Integrative Biosciences (MCIBS) Graduate Program, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

Journal of Micromechanics and Microengineering : Structures, Devices, and Systems

|August 12, 2017

Summary

This summary is machine-generated.

This study presents a novel acoustofluidic device for efficiently separating bacteria from blood. This technology enables label-free detection, improving sepsis diagnosis accuracy.

Keywords:

Acoustofluidics bacterial separation standing surface acoustic wave (SSAW)

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Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

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Published on: May 14, 2020

Area of Science:

Biomedical Engineering
Microfluidics
Acoustofluidics

Background:

Accurate bacterial identification in blood is crucial for timely sepsis diagnosis.
Current methods often require complex sample preparation and can be time-consuming.
Label-free separation techniques are needed to simplify diagnostics and reduce costs.

Purpose of the Study:

To develop and demonstrate an acoustofluidic device for label-free separation of bacteria from human blood.
To utilize acoustic radiation force for size-based separation of bacteria from blood cells.
To enable sensitive and specific detection of separated bacteria for improved sepsis diagnosis.

Main Methods:

Acoustofluidic device utilizing a tilted-angle standing surface acoustic wave (taSSAW) field.
Separation of *E. coli* from human blood cells based on size differences.
Flow cytometry for purity analysis and label-free electrochemical detection of separated bacteria.

Main Results:

Achieved over 96% purity in separating *E. coli* from red blood cells (RBCs).
Demonstrated reduced non-specific signals in electrochemical detection due to efficient blood cell removal.
Validated the effectiveness of the acoustofluidic platform for bacterial separation.

Conclusions:

The developed acoustofluidic platform offers label-free, high-purity bacterial separation from blood.
The technology exhibits high biocompatibility, flexibility, and potential for miniaturization and automation.
Integration with on-chip sensors can lead to point-of-care (POC) sepsis diagnostic devices.