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Effects of MicroRNA-27a on Myogenin Expression and Akt/FoxO1 Signal Pathway during Porcine Myoblast Differentiation

Shurun Zhang1, Xiaoling Chen1, Zhiqing Huang1

  • 1a Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu , Sichuan , P. R. China.

Animal Biotechnology
|August 12, 2017
PubMed

Insights

MicroRNA-27a (miR-27a) regulates skeletal muscle cell differentiation by inhibiting myogenin expression. This microRNA also impacts the Akt/FoxO1 signaling pathway, influencing porcine myoblast differentiation processes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Skeletal myoblast differentiation is a complex process regulated by transcription factors and signaling pathways.
  • Myogenin is a key regulator for initiating and maintaining myoblast differentiation.
  • The Akt/FoxO1 signaling pathway is crucial for skeletal muscle cell development.

Purpose of the Study:

  • To investigate the role of microRNA-27a (miR-27a) in porcine myoblast differentiation.
  • To determine the effects of miR-27a on myogenin expression.
  • To elucidate the impact of miR-27a on the Akt/FoxO1 signaling pathway.

Main Methods:

  • Quantitative real-time PCR to measure mRNA expression.
  • Western blotting to assess protein levels.
  • Manipulation of miR-27a levels through overexpression and inhibition.

Main Results:

  • miR-27a expression decreased during early differentiation and then increased.
  • Overexpression of miR-27a suppressed myogenin mRNA and protein levels.
  • Inhibition of miR-27a promoted myogenin expression.
  • miR-27a modulated the phosphorylation of Akt and the protein level of FoxO1.

Conclusions:

  • miR-27a acts as an inhibitor of myogenin expression in porcine myoblasts.
  • miR-27a influences porcine myoblast differentiation by regulating the Akt/FoxO1 signaling pathway.
Keywords:
Akt/FoxO1 signal pathwaymiR-27amyogeninporcine myoblast differentiation

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