Related Experiment Video
Updated: Feb 24, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
Published on: January 7, 2019
Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication
Maria Veronica Lopez1, Eduardo G Cafferata2, Diego L Viale2
1Laboratory of Molecular and Cellular Therapy, Leloir Institue-Conicet, Av Patricias Argentinas, 435, Buenos Aires, Argentina. mlopez@leloir.org.ar.
Abstract:
Here we describe a collection of methods that have been adapted to isolate and modify tumor-specific promoters (TSPs ) to drive viral replication for cancer therapy and other uses. We will describe as examples the secreted protein acidic and rich in cysteine (SPARC ) and the protease-activated receptor-1 (PAR-1) promoter. We outline strategies to select appropriate TSPs using bioinformatics resources and the methods utilized in their subsequent cloning, assessment of transcriptional activity, and their use in conditionally replicative oncolytic adenoviruses .
Related Concept Videos
Mechanisms of Retrovirus-induced Cancers
RNA Polymerase II Accessory Proteins
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
The Eukaryotic Promoter Region
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Prokaryotic Transcriptional Activators and Repressors

