Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.5K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.5K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.3K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.3K
Sampling Methods: Overview01:06

Sampling Methods: Overview

3.6K
A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling. 
In analytical chemistry, the choice of...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing Surface Binding Events on Golgi Microsomes Isolated from Cancer Cells by Capillary Electrophoresis.

Analytical chemistry·2026
Same author

Stearoyl-CoA Desaturase-1 Drives Tumor Growth by Interacting With Histone Deacetylase-2 and Deacetylating Nucleophosmin-1.

MedComm·2026
Same author

Intact Proteoform Analysis by Capillary Electrophoresis-Mass Spectrometry. Are We There Yet?

Angewandte Chemie (International ed. in English)·2025
Same author

Isoelectric point determination by icIEF as quality control for structural and functional characterization of HPV16 virus-like particles.

Analytica chimica acta·2025
Same author

Reduced RhoGDI2 Expression Disrupts Centrosome Functions and Promotes Mitotic Errors.

Cells·2025
Same author

Unveiling the enzymatic activity of a dimeric LDH isoform and its implications for allosteric inhibition strategies.

Protein science : a publication of the Protein Society·2025
Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Untargeted metabolomics, GC-MS and intelligent sensory analysis reveal wine-thermal induced alterations in chemical, sensory and metabolic bioactivities of Curculigo rhizome.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

A single bioanalytical LC-MS/MS method for the quantification of pelitinib across biological matrices: An insight into in vitro metabolic stability, in vivo pharmacokinetics, and IVIVE.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Multimodal analytical strategy for revealing the related compounds of color variation in Salvia miltiorrhiza after sweating.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Development and validation of a UPLC-MS/MS method for real-time neuropharmacokinetic monitoring of iboga alkaloids in rat brain.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

QC friendly polysorbate quantification in high concentration protein samples.

Journal of pharmaceutical and biomedical analysis·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
10:21

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

3.0K

Volumetric absorptive microsampling: Current advances and applications.

Miranda G M Kok1, Marianne Fillet1

  • 1Laboratory for the Analysis of Medicines, Centre for Interdisciplinary Research on Medicines (CIRM), University of Liège, Quartier Hopital, Avenue Hippocrate 15, 4000, Liège, Belgium.

Journal of Pharmaceutical and Biomedical Analysis
|August 15, 2017
PubMed
Summary
This summary is machine-generated.

Volumetric absorptive microsampling (VAMS) offers a precise method for collecting small blood volumes, simplifying analysis and storage. This technique demonstrates stability for various compounds, proving useful in pharmacokinetic and drug monitoring studies.

Keywords:
ApplicationsHematocritSample preparationStabilityVolumetric absorptive microsampling

More Related Videos

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.8K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
10:21

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

3.0K
A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.8K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.5K

Area of Science:

  • Biomedical engineering
  • Analytical chemistry
  • Pharmacology

Background:

  • Volumetric absorptive microsampling (VAMS) is a novel technique for biological fluid sampling, specifically whole blood.
  • It utilizes a porous hydrophilic tip to collect precise blood volumes (10 or 20μL) independent of hematocrit levels.

Purpose of the Study:

  • To review the general aspects of VAMS technology.
  • To demonstrate the applicability of VAMS through examples in pharmacokinetic and therapeutic drug monitoring studies.

Main Methods:

  • VAMS involves collecting small, accurate blood volumes on a specialized tip.
  • Dried samples are stable for storage and can be directly analyzed after extraction.
  • Optimal extraction conditions are determined using a design of experiments approach.

Main Results:

  • VAMS allows for hematocrit-independent blood volume collection.
  • Compound stability in dried VAMS samples varies but is enhanced at lower temperatures.
  • Extraction and direct analysis of compounds from the VAMS tip are feasible.

Conclusions:

  • VAMS is a versatile microsampling technique applicable to various biological fluids, particularly whole blood.
  • The method simplifies sample preparation and analysis, with potential applications in pharmacokinetic and therapeutic drug monitoring.
  • Further optimization of extraction conditions may be necessary for specific analytes and matrices.