Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

4.8K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.8K
Meiosis II02:02

Meiosis II

48.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
48.9K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.3K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.3K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

5.2K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.2K
Cohesins02:20

Cohesins

5.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.4K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

69.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
69.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing Surface Binding Events on Golgi Microsomes Isolated from Cancer Cells by Capillary Electrophoresis.

Analytical chemistry·2026
Same author

Stearoyl-CoA Desaturase-1 Drives Tumor Growth by Interacting With Histone Deacetylase-2 and Deacetylating Nucleophosmin-1.

MedComm·2026
Same author

Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.

Nature communications·2026
Same author

Beyond the stop: Oxadiazole TRIDs restore LRBA protein expression in nonsense-driven primary immunodeficiency.

Molecular therapy. Nucleic acids·2026
Same author

ADAMTS2: More than a procollagen N-proteinase.

Genes & diseases·2025
Same author

The ADAMTS2 metalloproteinase inhibits tumor growth by regulating the innate immune system.

Cancer cell international·2025
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
Same journal

PACAP and Maxadilan (PAC1 Agonist) Influence Plaque Progression, Migratory Ability, and Mitochondrial Morphology and Dynamics in Vascular Smooth Muscle Cells.

Cells·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K

Reduced RhoGDI2 Expression Disrupts Centrosome Functions and Promotes Mitotic Errors.

Mudrika Tripathi1, Nancy Garbacki1, Jérôme Willems1

  • 1Laboratory of Connective Tissues Biology, GIGA-Cancer, University of Liège, 4000 Liège, Belgium.

Cells
|November 26, 2025
PubMed
Summary
This summary is machine-generated.

RhoGDI2 (Rho Guanine Dissociation Inhibitor 2) regulates cell proliferation and centrosome function in cancer. Silencing RhoGDI2 impairs cancer cell killing by NK cells, revealing its dual role in cancer progression and immunity.

Keywords:
RhoGTPasescentrosomeschromosome congressionimmune synapseprimary cilia

More Related Videos

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
07:06

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

Published on: June 23, 2023

4.2K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.7K

Related Experiment Videos

Last Updated: Jan 10, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K
Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
07:06

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

Published on: June 23, 2023

4.2K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.7K

Area of Science:

  • Cell Biology
  • Cancer Biology
  • Immunology

Background:

  • RhoGDI2 is a Rho GTPase regulator involved in cytoskeleton organization and cell survival.
  • Its expression varies in human cancers, correlating with prognosis.
  • RhoGDI2 is highly expressed in immune cells, with similarities between primary cilia and immune synapses.

Purpose of the Study:

  • To investigate the mechanisms underlying RhoGDI2's dual role in cancer.
  • To explore RhoGDI2's function in cancer cell proliferation, centrosome regulation, and immune cell activity.

Main Methods:

  • Knockdown of RhoGDI2 expression in human cancer cell lines.
  • Assessment of proliferation rates, centrosome number, and ciliogenesis.
  • Silencing of RhoA to investigate GTPase involvement.
  • Silencing of RhoGDI2 in NK cells to evaluate immune synapse function.

Main Results:

  • RhoGDI2 knockdown reduced cancer cell proliferation, induced supernumerary centrosomes, and inhibited ciliogenesis.
  • RhoA silencing partially rescued centrosome and ciliary defects caused by RhoGDI2 suppression.
  • RhoGDI2 silencing in NK cells significantly impaired their cancer cell-killing activity via immune synapses.

Conclusions:

  • RhoGDI2 plays novel roles in centrosome function within human cancers.
  • RhoGDI2 is crucial for immune synapse function in immune cells, impacting anti-cancer activity.
  • These findings explain the observed dual role of RhoGDI2 in cancer progression and immunity.