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Updated: Feb 24, 2026

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
Published on: August 10, 2015
Decellularization of Liver and Organogenesis in Rats
Nana Shirakigawa1, Hiroyuki Ijima2
1Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Abstract:
Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 107 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.
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