Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

6.6K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
6.6K
Centrosome Duplication02:25

Centrosome Duplication

5.1K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
5.1K
Spindle Assembly02:50

Spindle Assembly

4.4K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.4K
The Mitotic Spindle02:27

The Mitotic Spindle

8.2K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
8.2K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

4.0K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
4.0K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

4.1K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

Septin-mediated coupling of protein import and division during chloroplast evolution.

bioRxiv : the preprint server for biology·2026
Same author

Microscopy Nodes: versatile 3D microscopy visualization with Blender.

EMBO reports·2026
Same author

Critical constituents and assembly principles of centriole biogenesis in human cells.

Nature reviews. Molecular cell biology·2025
Same author

C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1.

PLoS genetics·2025

Related Experiment Video

Updated: Feb 24, 2026

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

10.1K

Centriole Biogenesis: From Identifying the Characters to Understanding the Plot.

Niccolò Banterle1, Pierre Gönczy1

  • 1Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), CH-1015, Lausanne, Switzerland;

Annual Review of Cell and Developmental Biology
|August 17, 2017
PubMed
Summary

Centriole biogenesis, the assembly of microtubule organelles, is key for cell functions. This review details how specific proteins orchestrate centriole formation, providing insights into organelle biogenesis principles.

Keywords:
architectureassemblycartwheelcentriolelength control

More Related Videos

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.8K
Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

10.1K
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.8K
Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

16.5K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Centrioles are vital microtubule-based organelles essential for cellular polarity, motility, and division.
  • Understanding centriole biogenesis offers a model for eukaryotic organelle assembly.
  • Core proteins are known to be involved in centriole formation, but their precise roles require further elucidation.

Purpose of the Study:

  • To review current knowledge on the mechanisms of centriole biogenesis.
  • To elucidate the ordered functions of core proteins in centriole assembly.
  • To highlight fundamental principles of organelle biogenesis through the lens of centriole formation.

Main Methods:

  • Literature review of existing research on centriole biogenesis.
  • Analysis of the functional roles of core proteins in centriole assembly.
  • Synthesis of current understanding regarding protein scaffolding, initiation, and execution of centriole formation.

Main Results:

  • Centriole assembly involves a precise sequence of events orchestrated by specific core proteins.
  • The correct localization and timing of protein function are critical for successful centriole formation.
  • This process exemplifies fundamental principles applicable to the broader field of organelle biogenesis.

Conclusions:

  • Centriole biogenesis is a complex, highly regulated process crucial for cellular function.
  • The coordinated action of core proteins is essential for scaffolding, initiating, and executing centriole assembly.
  • Studying centriole formation provides valuable insights into the fundamental mechanisms governing eukaryotic organelle biogenesis.