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Related Experiment Video

Updated: Feb 24, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
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Nanoparticle-Mediated Recombinase Delivery into Maize.

Susana Martin-Ortigosa1,2,3, Brian G Trewyn4,5,6, Kan Wang7,8

  • 1Department of Agronomy, Iowa State University, Ames, IA, 50011-1010, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 18, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a DNA-free method for plant genome editing using gold-plated mesoporous silica nanoparticles (Au-MSNs) to deliver Cre recombinase protein into maize. This innovative nanomaterial system achieves efficient recombination, enabling the development of edited, fertile plants.

Keywords:
Cre/loxPGenome-editingIntracellular deliveryMesoporous silica nanoparticleZea mays

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Area of Science:

  • Plant Biotechnology
  • Nanotechnology in Agriculture
  • Molecular Biology

Background:

  • Traditional plant genome editing relies on DNA delivery methods, which can be inefficient and raise regulatory concerns.
  • Developing DNA-free delivery systems is crucial for advancing precision agriculture and crop improvement.

Purpose of the Study:

  • To present a novel, DNA-free method for delivering Cre recombinase protein into maize using gold-plated mesoporous silica nanoparticles (Au-MSNs).
  • To demonstrate the efficiency of this nanomaterial-mediated system for achieving targeted gene recombination in maize.

Main Methods:

  • Loading Cre recombinase protein into the pores of Au-MSNs.
  • Delivering Au-MSNs loaded with Cre protein into immature maize embryos (Lox-corn) via biolistic bombardment.
  • Utilizing Lox-corn lines with loxP sites flanking selection and reporter genes for recombination events.

Main Results:

  • Successful delivery and intracellular release of functional Cre recombinase protein within maize cells.
  • Achieved recombination at loxP sites, leading to the elimination of targeted genes.
  • As high as 20% of bombarded embryos yielded regenerable recombinant callus events, demonstrating significant efficiency.

Conclusions:

  • The described nanomaterial-mediated, DNA-free methodology offers a promising alternative for plant genome editing.
  • This approach has the potential to become an effective tool for precise crop improvement and genetic modification in plants.