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Updated: Feb 24, 2026

Production of Double-stranded DNA Ministrings
Published on: February 29, 2016
Production of Minicircle DNA Vectors Using Site-Specific Recombinases
Nafiseh Sanei Ata-Abadi1,2, Naeimeh Rezaei3, Kianoush Dormiani3
1Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. nafiseh.sanei@gmail.com.
Abstract:
Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in high-level and persistent expression of transgene in comparison with conventional plasmids and provides promising vehicles for gene therapy and vaccination. Here we describe the production of replicative minicircle DNA vectors using the PBAD/araC system expressing ΦC31 integrase in E. coli.
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