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Updated: Feb 24, 2026
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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
Published on: March 6, 2015
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Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues
Soojung Hahn1, Jongman Yoo2,3
1Department of Microbiology and Institute of Basic Medical Sciences, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do, 13488, South Korea.
Methods in Molecular Biology (Clifton, N.J.)
|August 18, 2017
Summary
Human gastrointestinal organoids, derived from stem cells, mimic organ function and are valuable for disease modeling. This protocol details creating these organoids from stomach or colon tissue.
Area of Science:
- Stem Cell Biology
- Gastroenterology
- Organoid Technology
Background:
- Organoids are 3D in vitro cultures from self-organizing stem cells, replicating organ structure and function.
- Human gastrointestinal epithelial organoids are generated from isolated intestinal crypts or gastric glands.
- These organoids recapitulate in vivo human physiology, containing differentiated and proliferated intestinal epithelial cell types.
Purpose of the Study:
- To describe a protocol for establishing human gastrointestinal organoids (gastroids and colonoids).
- To enable the generation of disease models using these organoids.
Main Methods:
- Isolation of glands or crypts from surgical stomach or colon tissue.
- Establishment of isolated tissues into three-dimensionally cultured organoids in vitro.
Main Results:
- Successful generation of human gastrointestinal epithelial organoids (gastroids and colonoids).
- Demonstration of organoid recapitulation of in vivo human gastrointestinal physiology.
Conclusions:
- Gastrointestinal organoids are effective tools for developing disease models.
- The described protocol provides a method for creating patient-specific gastrointestinal organoids for research and clinical applications.
