Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.3K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.3K
Autophagy01:27

Autophagy

6.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adipocyte Pten Inhibition Improves Metabolic Health Associated with Expanded Lipid Storage Capacity and Reduced Inflammation.

bioRxiv : the preprint server for biology·2026
Same author

Gridless DOA Estimator for 1.5-Bit Sparse Massive MIMO Systems Based on Covariance Matrix Estimation.

Entropy (Basel, Switzerland)·2026
Same author

Reanalysis data fusion-based tropical cyclone formation prediction network.

Scientific reports·2026
Same author

Development and properties of composite coatings for surface repair of cement concrete pavements.

Scientific reports·2026
Same author

Moderate iron restriction improves metabolism via epigenetic regulation of GDF15.

The Journal of nutritional biochemistry·2026
Same author

Retinoic acid impairs the osteogenic differentiation of BMSCs via the miR-486/FOXO1 axis: a therapeutic target for bone loss.

Biochemical pharmacology·2026

Related Experiment Video

Updated: Feb 24, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

2.4K

Autophagy in Adipocyte Differentiation.

Zhipeng Tao1, Longhua Liu1,2, Louise D Zheng1

  • 1Department of Human Nutrition, Foods, and Exercise, Fralin Translational Obesity Research Center, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 18, 2017
PubMed
Summary
This summary is machine-generated.

Autophagy is crucial for adipocyte differentiation and metabolic health. This study details methods to assess autophagy activity in adipocytes, highlighting the importance of autophagy flux over protein levels for accurate evaluation.

Keywords:
Adipocyte differentiationAdipose development and functionAutophagyEndocrineMetabolism

More Related Videos

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.8K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K

Related Experiment Videos

Last Updated: Feb 24, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

2.4K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.8K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K

Area of Science:

  • Metabolic Biology
  • Cellular Biology
  • Molecular Biology

Background:

  • Adipose tissue is vital for metabolic homeostasis, functioning as an endocrine organ and energy store.
  • Adipocyte differentiation, regulated by autophagy, is essential for adipose tissue development and function.
  • Autophagy deficiency impairs adipocyte differentiation, adipose tissue development, and adipokine secretion, potentially leading to severe health issues.

Purpose of the Study:

  • To describe a procedure for analyzing autophagy during adipocyte differentiation.
  • To compare the utility of steady-state autophagy protein levels versus autophagy flux in assessing autophagy activity in adipocytes.

Main Methods:

  • Detailed protocol for autophagy analysis in differentiating adipocytes.
  • Measurement of steady-state levels of key autophagy proteins (beclin 1, LC3, p62).
  • Assessment of autophagy flux to determine autophagic activity.

Main Results:

  • Autophagy plays a critical role in adipocyte differentiation and adipose tissue maintenance.
  • Accurate assessment of autophagy is essential for understanding metabolic diseases.
  • Autophagy flux provides a more robust measure of autophagy activity than steady-state protein levels.

Conclusions:

  • The presented methods enable accurate evaluation of autophagy in adipocytes.
  • Understanding autophagy dynamics is crucial for adipose biology and metabolic disease research.
  • Distinguishing between autophagy protein levels and flux is key for reliable autophagy assessment.