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Destabilizing the interplay between miR-1275 and IGF2BPs by Tamarix articulata and quercetin in hepatocellular

Yasmin M Shaalan1, H Handoussa1, R A Youness1

  • 1a Faculty of Pharmacy and Biotechnology, Pharmaceutical Biology Department , German University in Cairo , Cairo , Egypt.

Natural Product Research
|August 19, 2017
PubMed

Insights

Tamarix articulata, quercetin, and epigallocatechin gallate (EGCG) show promise for hepatocellular carcinoma (HCC) therapy. These phytochemicals enhance miR-1275 and suppress oncogenic IGF2BPs, targeting the miR-1275/IGF axis.

Area of Science:

  • Molecular Oncology
  • Phytochemistry
  • Epigenetics

Background:

  • Insulin-like growth factor-2 binding proteins (IGF2BPs) are oncogenic, upregulated in hepatocellular carcinoma (HCC), and targeted by miR-1275.
  • Plant-derived compounds are a significant source of anticancer drugs, potentially acting via miRNA modulation.

Purpose of the Study:

  • To investigate the potential of Tamarix articulata, quercetin, and epigallocatechin gallate (EGCG) in HCC treatment.
  • To explore how these phytochemicals epigenetically modulate the Insulin-like Growth Factor (IGF) axis through miR-1275.

Main Methods:

  • Treatment of Huh-7 HCC cells with Tamarix articulata ethyl acetate fraction, quercetin, and EGCG.
  • Assessment of cell viability.
  • Quantification of miR-1275, IGF2BP1, and IGF2BP3 mRNA expression levels.

Main Results:

  • Tamarix articulata ethyl acetate fraction significantly reduced Huh-7 cell viability.
  • All tested phytochemicals significantly enhanced miR-1275 expression.
  • Phytochemical treatments suppressed IGF2BP1 and IGF2BP3 mRNA levels.

Conclusions:

  • Tamarix articulata, quercetin, and EGCG demonstrate potential for molecular-targeted HCC therapy.
  • These phytochemicals disrupt the interaction between miR-1275 and the IGF axis, offering a novel therapeutic strategy.
Keywords:
EGCGIGF2BPsTamarixhepatocellular carcinomamiRNAsquercetin

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