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Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
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Anodization of magnesium for biomedical applications - Processing, characterization, degradation and
Aaron F Cipriano1, Jiajia Lin1, Christopher Miller2
1Department of Bioengineering, University of California, Riverside, CA 92521, USA; Materials Science & Engineering, University of California, Riverside, CA 92521, USA.
Acta Biomaterialia
|August 19, 2017
Summary
Anodic oxidation of magnesium (Mg) in KOH electrolyte creates a modified surface for bioresorbable implants. This process enhances Mg-based material degradation control and influences bone marrow derived mesenchymal stem cell responses.
Area of Science:
- Biomaterials Science
- Materials Engineering
- Biomedical Engineering
Background:
- Magnesium (Mg)-based biomaterials are explored for biodegradable implants.
- Controlling Mg degradation rate is crucial for medical applications.
- Anodization offers a method to modify Mg surface properties.
Purpose of the Study:
- To investigate anodization of Mg in KOH electrolyte for bioresorbable implant applications.
- To evaluate the effects of anodization on surface, degradation, and biological properties.
- To assess the reproducibility and significance of the anodization process.
Main Methods:
- Anodization of Mg using KOH electrolyte at varying potentials (1.8, 1.9, 2.0V).
- Annealing of anodized samples (1.9 AA sample).
- Electrochemical testing for corrosion current density.
- In vitro culture with bone marrow derived mesenchymal stem cells (BMSCs) under direct and indirect contact.
Main Results:
- The 1.9 AA sample exhibited homogenous surface microstructure and composition.
- Reduced corrosion current density and a distinct degradation mode (passivation layer growth) were observed for the 1.9 AA sample.
- The 1.9 AA sample showed no adverse effect on BMSC adhesion/morphology under indirect contact but reduced cell spreading under direct contact.
Conclusions:
- Anodic oxidation can modulate Mg-based biomaterial degradation and BMSC responses.
- The 1.9 AA sample demonstrates potential for bioresorbable implant applications.
- Direct culture methods are valuable for assessing Mg-based biomaterial cytocompatibility.
