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Updated: Jul 29, 2026

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
Published on: September 7, 2009
The step-wise framework to design a chromatography-based hydrophobicity assay for viral particles
Sarah A Johnson1, Alison Walsh2, Matthew R Brown1
1DBRRII, Office of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, 20993, USA.
Abstract:
A high-salt, hydrophobic interaction chromatography (HIC) method was developed to measure the relative hydrophobicity of a diverse set of solutes. Through the careful control of buffer pH and salt concentration, this assay was then used to ascertain for the first time the relative hydrophobicity values of three different bacteriophage, four mammalian viruses, and a range of biotech medicinal proteins as benchmarked to protein standards previously characterized for hydrophobicity.
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