Related Experiment Video
Updated: Feb 24, 2026
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Published on: March 10, 2017
Draft Genome Sequence of Staphylococcus aureus S681, a Tetracycline-Sensitive Livestock-Associated
Marc J A Stevens1,2, Roger Stephan1, Sophia Johler3
1Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
Abstract:
We present the draft genome sequence of an atypical tetracycline-susceptible livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) strain. It contains 2,817,340 bp and 2,858 coding sequences, including 6 rRNA operons, 56 tRNAs, and 4 noncoding RNA (ncRNA) genes. The strain harbors a tet(M) gene, but 15 point mutations in amino acids are present that likely impair the functionality of TetM.
Insights
We sequenced an atypical livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) strain. Despite having the tet(M) gene, mutations likely prevent tetracycline resistance, making it susceptible.
Area of Science:
- Microbiology
- Genomics
- Antimicrobial Resistance
Background:
- Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) poses a significant One Health challenge.
- Understanding the genetic basis of antibiotic resistance in LA-MRSA is crucial for effective control strategies.
Purpose of the Study:
- To present the draft genome sequence of an unusual tetracycline-susceptible LA-MRSA strain.
- To investigate the genetic factors potentially contributing to its atypical resistance profile.
Main Methods:
- Whole-genome sequencing of the MRSA strain.
- Bioinformatic analysis to identify genes, operons, and mutations.
- Comparative genomics to understand the tet(M) gene context.
Main Results:
- The draft genome sequence comprises 2,817,340 base pairs with 2,858 coding sequences.
- The strain possesses the tet(M) gene, conferring tetracycline resistance.
- Fifteen specific amino acid point mutations were identified within the TetM protein, likely impairing its function.
Conclusions:
- This study provides the genome sequence of a unique tetracycline-susceptible LA-MRSA.
- The identified mutations in the tet(M) gene offer a potential explanation for the observed susceptibility.
- Further research is needed to confirm the functional impact of these mutations on tetracycline resistance.
