Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system...

Area of Science:

• Biotechnology
• Materials Science
• Tissue Engineering

Background:

• Three-dimensional (3D) cell printing enables precise deposition of cells into 3D constructs.
• Hydrogel bioinks are widely used but have limitations in crosslinking and rheology for precise 3D shaping.
• Decellularized extracellular matrix (dECM) offers potential as a bioink material.

Purpose of the Study:

• To investigate factors influencing cell printing with dECM bioinks.
• To develop and evaluate a novel 3D cell printing system with integrated heating modules.
• To enhance the precise stacking and gelation of dECM-based 3D cell-laden constructs.

Main Methods:

• Investigated bioink concentration, viscosity, and crosslinking effects on printability.
• Developed a 3D cell printing system with heating modules for simultaneous gelation at 37°C.
• Performed mechanical testing and 3D construct stacking analyses to validate performance.

Main Results:

• The heating system significantly improved the printability and precise stacking of dECM bioinks compared to a non-heating system.
• Simultaneous gelation during printing was achieved using the heating module, facilitated by the 37°C gelation property of pH-adjusted dECM bioink.
• Mechanical testing and stacking analyses confirmed enhanced construct integrity and precision.
• The heating system did not induce negative effects, such as cell death, on the printed cells.

Conclusions:

• The developed 3D cell printing system with heating modules enhances the precise stacking of dECM bioinks.
• This technology facilitates simultaneous gelation, improving printability and construct fidelity.
• The findings support the application of this 3D bioprinting system in tissue engineering and drug development.