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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Isolation of Human BAMBIhighMFGE8high Umbilical Cord-Derived Mesenchymal Stromal Cells
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Mesenchymal stromal/stem cell separation methods: concise review.

Andreas Nicodemou1, Lubos Danisovic2,3

  • 1Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia.

Cell and Tissue Banking
|August 20, 2017
PubMed
Summary

Mesenchymal stem cells (MSCs) are vital for regenerative medicine due to their self-renewal and migration abilities. This review details MSC separation techniques and markers for clinical applications.

Keywords:
MACS, FACS, surface antigensMesenchymal stem (stromal) cellsSeparation techniques

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Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Biotechnology

Background:

  • Mesenchymal stem cells (MSCs) exhibit plasticity, self-renewal, and bioactive molecule secretion, making them valuable for regenerative medicine.
  • MSCs can be isolated from various sources like bone marrow, umbilical cord, and adipose tissue.
  • Efficient separation of MSCs is critical before in vitro expansion, banking, or clinical use.

Purpose of the Study:

  • To review current mesenchymal stem cell (MSC) separation techniques.
  • To outline the principles, advantages, and limitations of these separation methods.
  • To discuss markers used for immunoaffinity-based MSC separation.

Main Methods:

  • Review of existing literature on MSC separation techniques.
  • Analysis of principles behind various separation methods.
  • Examination of immunoaffinity markers for MSC isolation.

Main Results:

  • Various MSC separation techniques are available, each with distinct principles, benefits, and drawbacks.
  • Immunoaffinity separation relies on specific markers for isolating MSCs.
  • The choice of separation method impacts MSC yield and purity.

Conclusions:

  • Effective MSC separation is essential for successful regenerative medicine applications.
  • Understanding separation techniques and markers optimizes MSC isolation protocols.
  • Further research can refine MSC separation for enhanced therapeutic potential.