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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration
Roxana Khoshravesh1, Vanessa Lundsgaard-Nielsen1, Stefanie Sultmanis1
1Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St., Toronto, ON, Canada, M5S 3B2.
Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2017
Summary
This study details protocols for preparing plant tissues for high-resolution imaging. These methods link plant cell structure and physiology during photorespiration, impacting plant evolution and development.
Area of Science:
- Plant Biology
- Cellular Biology
- Microscopy Techniques
Background:
- Photorespiration significantly influences plant evolution and development.
- Understanding the link between subcellular structure and physiology during photorespiration is crucial.
- High-resolution imaging techniques are essential for visualizing these links.
Purpose of the Study:
- To provide established protocols for preparing plant tissues for high-resolution imaging.
- To guide researchers in using light microscopy, transmission electron microscopy, and immunohistochemistry.
- To enhance understanding of the theoretical concepts behind each preparation step.
Main Methods:
- Detailed protocols for plant tissue preparation for light and transmission electron microscopy.
- Established immunohistochemistry techniques for protein detection in plant tissues.
- Inclusion of theoretical concepts for tissue preservation, embedding, and staining.
Main Results:
- Established protocols enable high-resolution imaging of plant subcellular structures.
- Methods facilitate the detection of proteins crucial for understanding photorespiration.
- A protocol for quantifying cellular parameters from high-resolution images is provided.
Conclusions:
- The presented protocols are vital for researchers studying photorespiration and its effects.
- High-resolution imaging and immunohistochemistry provide crucial insights into plant physiology and evolution.
- Standardized methods aid in rigorous hypothesis testing related to plant cell biology.
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