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Updated: Feb 24, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
[Blood Transfusion Selection in Patients with Autoimmune Hemolytic Anemia Producing Class Antibodies]
Qin-Li Ding1, Bao-Long Chen1, Fang Qiu1
1Department of Blood Transfusion, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.
Objective:
To analyze the reasons for the incompatibility of cross matching in lymphoma patients complicated by autoimmune hemolytic anemia (AIHA), and to provide compatible blood to patients for multiple blood transfusion.
Methods:
The test tube method was used for routine identification of ABO blood group, Rh typing and direct anti-globulin test; the patients antibody was identified by saline water method, micro column gel card; and the saline water method, Polybrene method and antiglobulin method were used for blood cross matching test of the patients.
Results:
In the serum of the patient, positive autoantibodies were detected, in the elution, the Ce antibody was detected. After infusion of the corresponding antigen-negative blood, the patient's hemoglobin level was significantly improved.
Conclusion:
Only firstly accurately identifying antibodies existed in serum and elution, then selecting corresponding antigen negative blood, can ensure the safety and effectiveness of blood transfusion.
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