Related Experiment Video
Updated: Feb 24, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
An interactive website for analytical method comparison and bias estimation
Burak Bahar1, Ayse F Tuncel, Earle W Holmes2
1Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
Objectives:
Regulatory standards mandate laboratories to perform studies to ensure accuracy and reliability of their test results. Method comparison and bias estimation are important components of these studies.
Design & Methods:
We developed an interactive website for evaluating the relative performance of two analytical methods using R programming language tools. The website can be accessed at https://bahar.shinyapps.io/method_compare/.
Results:
The site has an easy-to-use interface that allows both copy-pasting and manual entry of data. It also allows selection of a regression model and creation of regression and difference plots. Available regression models include Ordinary Least Squares, Weighted-Ordinary Least Squares, Deming, Weighted-Deming, Passing-Bablok and Passing-Bablok for large datasets. The server processes the data and generates downloadable reports in PDF or HTML format.
Conclusions:
Our website provides clinical laboratories a practical way to assess the relative performance of two analytical methods.
Related Concept Videos
Development of Analytical Methods
Bias
In statistics, a sampling bias is created when a sample is collected from a population, and some members of the population are not as likely to be chosen as others (remember, each member...
Contaminants and Errors
Another key consideration is determining the appropriate number of samples required to...
Accuracy and Errors in Hypothesis Testing
In hypothesis testing, the probability of making a Type I error, denoted as α, is commonly set at 0.05. This significance level indicates a 5%...
Titrimetric Methods: Types and Commonly Used Strategies
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation
On...

