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Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
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A new photoactivatable near-infrared-emitting QCy7 fluorophore for single-molecule super-resolution microscopy
Dean-Paulos Klötzner1, Kathrin Klehs, Mike Heilemann
1Goethe University Frankfurt, Institute of Organic Chemistry and Chemical Biology, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany. heckel@uni-frankfurt.de.
Abstract:
A new photoactivatable fluorophore based on a quinone-cyanine-7 (QCy7) scaffold was synthesized and spectroscopically characterized. The fluorophore exhibits fluorescence in the near-infrared spectral window and a large Stokes shift. Its application in super-resolution microscopy as an antibody bioconjugate is demonstrated.

