Related Experiment Video

Updated: Feb 24, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

11.2K

A polycation coated liposome as efficient siRNA carrier to overcome multidrug resistance

Yuqiong Xia1, Xiaofei Wang1, He Cheng1

  • 1School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710126, China.

Colloids and Surfaces. B, Biointerfaces
|August 22, 2017
PubMed

Insights

This study introduces a novel, non-toxic poly(diallyldimethylammonium chloride) (PDADMAC) coated liposome for delivering multidrug resistance (MDR) siRNA and anticancer drugs, effectively inhibiting tumor growth and overcoming chemotherapy resistance.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Cancer Research

Background:

  • Multidrug resistance (MDR) significantly hinders effective cancer chemotherapy.
  • Codelivery of MDR1 siRNA and anticancer drugs shows potential for inhibiting tumor proliferation.

Purpose of the Study:

  • To develop and evaluate a novel poly(diallyldimethylammonium chloride) (PDADMAC) coated liposome (AL-PDAD-RNA) as a carrier for MDR1 siRNA.
  • To assess the efficacy of AL-PDAD-RNA in reversing doxorubicin resistance in OVCAR8/ADR cells.

Main Methods:

  • Synthesis of PDADMAC-coated liposomes (AL-PDAD) for siRNA loading.
  • Evaluation of siRNA loading capacity and release under physiological conditions.
  • In vitro assessment of doxorubicin resistance reversal and gene silencing efficacy compared to commercial agents.

Main Results:

  • AL-PDAD-RNA effectively loaded and released siRNA under physiological conditions.
  • Codelivery of doxorubicin and siRNA via AL-PDAD-RNA demonstrated improved tumor inhibition compared to free doxorubicin.
  • The gene silencing effect of AL-PDAD-RNA was comparable to lipofectamine but exhibited lower toxicity.

Conclusions:

  • PDADMAC-coated liposomes represent a simple, highly effective, and non-toxic siRNA carrier.
  • This novel carrier system shows promise for overcoming multidrug resistance in cancer therapy.
  • PDADMAC-coated liposomes have potential applications in siRNA and plasmid delivery for various therapeutic strategies.
Keywords:
LiposomeMultidrug resistancePolymer coatingsiRNA delivery

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

11.3K

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
57
Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
45
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.8K
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
263
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.4K