Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids01:21

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

838
Tetrahydrocannabinol (THC) is a phytocannabinoid that primarily interacts with the CB1 receptor, a type of G protein-coupled receptor (GPCR) predominantly in and around the chemoreceptor trigger zone (CTZ) and emetic center. THC also blocks the serotonin receptor activity in the dorsal vagal complex (DVC) by inhibiting serotonin release. THC exerts its anti-emetic effects through these interactions, which are beneficial for patients undergoing chemotherapy.
Two synthetic agonists of THC,...
838
Opioid Receptors: Overview01:22

Opioid Receptors: Overview

5.2K
Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...
5.2K
Desensitization and Tachyphylaxis01:20

Desensitization and Tachyphylaxis

3.2K
Tachyphylaxis is described as a rapid decrease in response to a drug after repeated or continuous administration of the same drug dose. It is a phenomenon where the body becomes less responsive to a particular substance or intervention over time, requiring higher doses or stronger interventions to achieve the same effect. It results from adaptive changes in the body's receptors, signaling pathways, or physiological processes that occur in response to prolonged exposure to a stimulus.
3.2K
GPCR Desensitization01:12

GPCR Desensitization

8.4K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
8.4K
Neurochemical Transmission: Sites of Drug Action01:26

Neurochemical Transmission: Sites of Drug Action

3.7K
Neurochemical transmission, the conduction of electrical impulses between neurons mediated by neurotransmitters, plays a vital role in various physiological processes. Autonomic drugs exert their effects by modulating neurotransmission within the autonomic nervous system. For instance, drugs such as hemicholinium block the precursor uptake necessary for synthesizing acetylcholine, an essential autonomic neurotransmitter. Following synthesis, neurotransmitters are stored in vesicles. Metyrosine...
3.7K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.3K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of Mobile Health Augmented Cardiac Rehabilitation on Clinical Outcomes among Post-Acute Coronary Syndrome Patients: A Randomized Controlled Trial.

Pakistan journal of medical sciences·2026
Same author

Discovery of lipid biomarkers for idiopathic Parkinson's disease in red blood cells and plasma.

NPJ Parkinson's disease·2026
Same author

Retrospective case-control study of pre-diagnosis observational and prescription data in Parkinson's disease.

Scientific reports·2026
Same author

International Union of Basic and Clinical Pharmacology. CXXII. Applying an objective evaluation to the status of class A orphan G protein-coupled receptors.

Pharmacological reviews·2026
Same author

How feasible is self-monitoring for the management of prehypertension? The REVERSE study.

The British journal of general practice : the journal of the Royal College of General Practitioners·2026
Same author

Are clinical improvements in anxiety or depression associated with improvement in walking fitness following a cardiac rehabilitation programme?

Open heart·2026
Same journal

Preface.

Advances in pharmacology (San Diego, Calif.)·2026
Same journal

Brain shuttle peptides: From permeability assay toolbox to data-driven discovery.

Advances in pharmacology (San Diego, Calif.)·2026
Same journal

Brain shuttle peptides derived from phage display.

Advances in pharmacology (San Diego, Calif.)·2026
Same journal

Physiological strategies for brain delivery.

Advances in pharmacology (San Diego, Calif.)·2026
Same journal

Brain shuttle peptides derived from natural proteins.

Advances in pharmacology (San Diego, Calif.)·2026
Same journal

In vitro blood-brain barrier models for the study of brain shuttle peptide transport.

Advances in pharmacology (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
14:25

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Published on: September 21, 2019

7.7K

Endocannabinoid Turnover.

Christopher J Fowler1, Patrick Doherty2, Stephen P H Alexander3

  • 1Umeå University, Umeå, Sweden.

Advances in Pharmacology (San Diego, Calif.)
|August 23, 2017
PubMed
Summary
This summary is machine-generated.

This review covers endocannabinoid metabolism, enzymes, and inhibitors. Targeting single enzymes for therapeutic use may not be the most effective strategy for the endocannabinoid system.

Keywords:
2-ArachidonoylglycerolAnandamideDiacylglycerol lipaseFatty acid amide hydrolaseMonoacylglycerol lipaseN-acylphosphatidylethanolamine phospholipase D

More Related Videos

Administration of Δ9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice
07:51

Administration of Δ9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice

Published on: August 1, 2025

1.1K
Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents
03:43

Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents

Published on: August 22, 2025

585

Related Experiment Videos

Last Updated: Feb 24, 2026

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
14:25

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Published on: September 21, 2019

7.7K
Administration of Δ9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice
07:51

Administration of Δ9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice

Published on: August 1, 2025

1.1K
Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents
03:43

Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents

Published on: August 22, 2025

585

Area of Science:

  • Biochemistry
  • Pharmacology
  • Neuroscience

Background:

  • The endocannabinoid system plays a crucial role in physiological processes.
  • Anandamide and 2-arachidonoylglycerol are key endocannabinoids.
  • Understanding their metabolism is vital for therapeutic development.

Purpose of the Study:

  • To review the metabolism of anandamide and 2-arachidonoylglycerol.
  • To identify associated enzymes, their characterization, and inhibitor profiles.
  • To evaluate the therapeutic potential of targeting the endocannabinoid system.

Main Methods:

  • Literature review of biosynthetic, hydrolytic, and oxidative pathways.
  • Analysis of enzyme kinetics and inhibitor studies.
  • Assessment of current clinical trial data for enzyme inhibitors.

Main Results:

  • Detailed description of enzymes involved in endocannabinoid metabolism.
  • Identification of specific inhibitors for key metabolic enzymes.
  • Limited success in therapeutic exploitation, with ongoing trials for fatty acid amide hydrolase inhibitors.

Conclusions:

  • Targeting single enzymes in the endocannabinoid system may have limited therapeutic success.
  • Further research is needed to explore alternative strategies for modulating endocannabinoid signaling.
  • The review provides a comprehensive overview of endocannabinoid metabolism and its therapeutic implications.