Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Premature termination of early-phase cancer clinical trials: "It's the economy, stupid!"

Journal of the National Cancer Institute·2026

Same author

An emerging family of immunogenic epitopes in Chagas disease.

Trends in parasitology·2026

Same author

Decoding and deciphering a subcellular ZIP code system.

Trends in cell biology·2026

Same author

Endoscopic liquid biopsies of gastric fluid in a large human patient cohort reveal DNA content as a candidate tumor biomarker in gastric cancer.

eLife·2025

Same author

Leishmania amazonensis impairs phagosome acidification in B-1 phagocytes as an unrecognized parasite infection and proliferation mechanism.

Scientific reports·2025

Same author

Matrix Metalloproteinases as Candidate Antigenic Determinants for Anti-Tumor Autoantibodies in Human Ovarian Cancer: A Post Hoc Analysis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Same journal

Preface.

Advances in pharmacology (San Diego, Calif.)·2026

Same journal

Brain shuttle peptides: From permeability assay toolbox to data-driven discovery.

Advances in pharmacology (San Diego, Calif.)·2026

Same journal

Physiological strategies for brain delivery.

Advances in pharmacology (San Diego, Calif.)·2026

Same journal

Brain shuttle peptides derived from natural proteins.

Advances in pharmacology (San Diego, Calif.)·2026

Same journal

In vitro blood-brain barrier models for the study of brain shuttle peptide transport.

Advances in pharmacology (San Diego, Calif.)·2026

Same journal

Polymeric nanoparticles functionalized with peptides: Advanced strategies for crossing the blood-brain barrier.

Advances in pharmacology (San Diego, Calif.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Brain shuttle peptides derived from phage display.

Alexander M Ille¹, Fenny H F Tang¹, Ethan R Chen¹

¹Rutgers Cancer Institute, Newark, NJ, United States; Division of Cancer Biology, Department of Radiation Oncology, Rutgers New Jersey Medical School, Newark, NJ, United States.

Advances in Pharmacology (San Diego, Calif.)

|June 7, 2026

Summary

This summary is machine-generated.

Phage display technology identifies peptides that target specific tissues, like the brain. These peptides cross the blood-brain barrier (BBB) for potential brain disorder therapies.

Keywords:

BBB Blood-brain barrier Brain targeting Phage display Vascular endothelium

More Related Videos

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Related Experiment Videos

Last Updated: Jun 9, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Area of Science:

Molecular biology
Neuroscience
Biotechnology

Background:

Vascular beds show molecular differences across tissues, including the brain.
Phage display is a technique used to discover peptide ligands for specific tissues.
Peptide ligands can facilitate targeted drug delivery across biological barriers.

Purpose of the Study:

To explore the use of in vivo phage display for discovering tissue-specific peptide ligands.
To identify peptides that can cross the blood-brain barrier (BBB).
To assess the potential of these peptides in preclinical therapeutic applications for brain disorders.

Main Methods:

In vivo phage display screening of peptide libraries.
In vitro phage display using models like BBB models, cultured cells, and immobilized receptors.
Identification and characterization of peptide-receptor interactions, such as the transferrin receptor (TfR) complex.

Main Results:

Discovery of the iron-mimicking peptide CRTIGPSVC, which binds to the transferrin/transferrin receptor (TfR) complex for BBB transport.
Identification of various other brain-homing and BBB-crossing peptides through phage display.
Demonstration of preclinical therapeutic applications for brain disorders using identified peptides.

Conclusions:

Phage display is effective in discovering peptides that target specific tissues and cross the BBB.
The identified peptides show promise for developing targeted brain therapies.
Further research using phage display will enhance understanding of BBB transport and advance brain-targeted treatments.