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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
Published on: October 17, 2022
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Red Blood Cells: A Source of Extracellular Vesicles
Winston Patrick Kuo1,2, John C Tigges3, Vasilis Toxavidis3
1CloudHealth Genomics, Ltd, Shanghai, China. winston.kuo@cloudhealth99.com.
Methods in Molecular Biology (Clifton, N.J.)
|August 23, 2017
Summary
Red blood cells (RBCs) release exosomes during development and extracellular vesicles (EVs) during aging. Both RBC exosomes and EVs are valuable sources for research.
Area of Science:
- Hematology
- Cell Biology
- Extracellular Vesicles
Background:
- Red blood cells (RBCs) undergo a lifespan and release various extracellular vesicles (EVs).
- Understanding the distinct origins and release mechanisms of RBC-derived EVs is crucial.
Purpose of the Study:
- To differentiate and describe the biogenesis of RBC exosomes and EVs.
- To highlight the potential of RBCs as a source for EVs in research.
Main Methods:
- Investigated RBC exosome formation during erythropoiesis in bone marrow.
- Analyzed RBC EV generation through plasma membrane budding in circulation.
- Examined the role of complement-mediated calcium influx in EV release.
Main Results:
- RBC exosomes originate from multivesicular bodies (MVBs) fusing with the plasma membrane during development.
- RBC EVs are formed by plasma membrane budding during RBC aging, triggered by calcium influx.
- Stored RBCs also serve as a source of these EVs.
Conclusions:
- RBC exosomes and EVs have distinct biogenesis pathways.
- RBCs and stored RBCs represent a consistent and accessible source of EVs for scientific investigation.
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