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Nanostructured biosensor using bioluminescence quenching technique for glucose detection.

Longyan Chen1, Longyi Chen1, Michelle Dotzert2

  • 1Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON, N6A 5B9, Canada.

Journal of Nanobiotechnology
|August 24, 2017
PubMed
Summary

This study presents a novel bioluminescence biosensor for glucose detection. The sensor uses gold nanoparticles and a luciferase protein to enable sensitive, non-invasive glucose monitoring without an external power source.

Keywords:
BioluminescenceBiosensorGlucoseNanoparticles

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Biosensing

Background:

  • Conventional glucose monitoring methods often require external energy sources, limiting in vivo applications.
  • Bioluminescence offers an alternative light emission mechanism, independent of external power, utilizing natural bioluminescent proteins.
  • Developing non-invasive and rapid glucose detection methods is crucial for effective health management.

Purpose of the Study:

  • To develop a nanostructured biosensor utilizing bioluminescence for rapid and non-invasive glucose detection.
  • To conjugate luciferase bioluminescence protein (Rluc) with β-cyclodextrin (β-CD) for glucose sensing.
  • To establish a sensitive and selective method for quantifying glucose concentrations.

Main Methods:

  • Conjugation of Rluc with β-CD and subsequent quenching of bioluminescence by gold nanoparticles (Au NPs).
  • Utilizing a competitive reaction where glucose displaces Au NPs, restoring Rluc bioluminescence.
  • Establishing a linear relationship between restored bioluminescence intensity and logarithmic glucose concentration (1-100 µM).
  • Evaluating sensor selectivity and performance in diabetic rat blood samples.

Main Results:

  • A novel bioluminescence-based glucose sensor was successfully developed.
  • The sensor demonstrated a linear response to glucose concentrations in the range of 1-100 µM.
  • The bioluminescence intensity of Rluc was quenched by Au NPs and restored in the presence of glucose.
  • The sensor showed good selectivity and was tested in biological samples.

Conclusions:

  • A sensitive bioluminescence sensor for rapid glucose detection has been designed.
  • The developed sensor offers a promising alternative for glucose monitoring, particularly for in vivo applications.
  • This approach highlights the potential of bioluminescence and nanotechnology in developing advanced biosensing platforms.