Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Protein Organization01:13

Protein Organization

159.2K
Overview
159.2K
Protein Organization01:24

Protein Organization

9.8K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
9.8K
Protein Folding01:25

Protein Folding

11.8K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
11.8K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.6K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.6K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

28.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Polymerization-Enabled Folding and Assembly to Chemical Evolution: Key Processes for Emergence of Functional Polymers in the Origin of Life.

Astrobiology·2025
Same author

Weak effects of prebiotically plausible peptides on self-triphosphorylation ribozyme function.

RSC chemical biology·2024
Same author

Alkyl nitrite-enabled palladium-catalyzed terminal selective oxidative cyclization of 4-penten-1-ols.

Chemical communications (Cambridge, England)·2024
Same author

Palladium/Iron-Catalyzed Wacker-Type Oxidation of Aliphatic Terminal and Internal Alkenes Using O<sub>2</sub>.

ACS omega·2023
Same author

Peptide conjugates with polyaromatic hydrocarbons can benefit the activity of catalytic RNAs.

Chemical science·2023
Same author

Robust and scalable single-molecule protein sequencing with fluorosequencing.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Feb 24, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

8.0K

Templated Self-Assembly of Dynamic Peptide Nucleic Acids.

John M Beierle1, Yasuyuki Ura1, M Reza Ghadiri1

  • 1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

Biochemistry
|August 24, 2017
PubMed
Summary
This summary is machine-generated.

Researchers studied template-directed dynamic assembly of thioester peptide nucleic acid (tPNA) using tPNA-DNA conjugates. This approach characterizes complex equilibria and mismatch correction in dynamic informational polymers.

More Related Videos

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.7K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

8.0K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.7K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.5K

Area of Science:

  • Biochemistry
  • Polymer Chemistry
  • Origins of Life Research

Background:

  • Template-directed synthesis is crucial for life.
  • Novel mechanisms for nucleic acid analogue assembly exist.
  • Characterizing dynamic templated reactions remains challenging.

Purpose of the Study:

  • To investigate template-directed dynamic assembly of thioester peptide nucleic acid (tPNA).
  • To develop methods for characterizing complex assembly equilibria in dynamic polymers.
  • To understand mismatch correction and fidelity in tPNA assembly.

Main Methods:

  • Designed and synthesized tPNA-DNA conjugates.
  • Utilized DNA primer for controlled tPNA backbone positioning.
  • Characterized dynamic nucleobase mismatch correction and assembly fidelity.
  • Conducted theoretical studies on hybridization affinity and fidelity.

Main Results:

  • Demonstrated controlled assembly and mismatch correction using tPNA-DNA conjugates.
  • Quantified the fidelity of dynamic assembly across the tPNA backbone.
  • Provided theoretical insights into fidelity based on hybridization affinity.
  • Established a new methodology for characterizing dynamic informational polymers.

Conclusions:

  • tPNA-DNA conjugates offer a robust method for studying dynamic polymer assembly.
  • Understanding fidelity is key for developing self-editing polymers and materials.
  • The methodology facilitates the study of complex chemical equilibria in dynamic systems.