Dynamics of regulatory T cells (T_regs ) in patients with oral squamous cell carcinoma

The immune dysfunction in oral squamous cell carcinoma (OSCC) patients is one of the major factors for growth and dissemination of tumor affecting disease-free survival.

The phenotypic and functional characteristics of Regulatory T (T_reg ) CD4⁺ CD25⁺ FoxP3⁺ subsets in OSCC patients were assessed by multicolor flow cytometry and its effector component (TGF-β) by Western blot and qRT-PCR.

An increased (P < 0.05) prevalence of T_reg phenotypes (CD4⁺ CD25⁺ , CD4⁺ FoxP3⁺ , CD8⁺ FoxP3⁺ , CD4⁺ CD25⁺ FoxP3⁺ ) was observed in the peripheral circulation of OSCC patients that positively correlated with clinicopathological features. The increased frequency of CD4⁺ CD8⁺ CD25⁺ FoxP3⁺ , a unique T cell subset, CTLA-4⁺ , GITR⁺ , NrP1⁺ , HLA-DR⁺ , CD127⁺ , Tbet⁺ , TGF-β⁺ , and granzyme B⁺ (GzmB) T_regs also showed a significantly higher prevalence in OSCC patients. Functionally, CD4⁺ FoxP3⁺ T_regs showed skewed expression of IL-2, IL-10, and IL-35 in patients as compared with the normal controls. Further, enhanced expression of CCR5 and CCR7 on T_regs with up regulation of their ligands (CCL5, CCL19, and CCL21) in tumor cells indicates efficient recruitment and trafficking of T_regs to the tumor site.

It seems reasonable to assume that modulation of functional dynamics of selective T_reg subsets may be useful in developing immunotherapeutic strategy for OSCC patients.