Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subviral Agents01:29

Subviral Agents

642
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
642
Pharmacokinetics: Drug–Food and Drug–Viral Interactions01:26

Pharmacokinetics: Drug–Food and Drug–Viral Interactions

306
A drug interaction occurs when the concurrent use of another drug, food, or an external substance alters the pharmacological activity of a drug. This interaction can modify the action of the original drug, affecting its effectiveness and safety.Drug–food interactions are significant as they impact drug absorption, metabolism, and excretion. For example, grapefruit juice is a well-known disruptor of drug metabolism. It inhibits the cytochrome P450 3A4 enzyme, crucial for the metabolism of...
306
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

560
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
560

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-mechanistic mitochondria-mediated N-methylated YKWYYRGAA-decorated nano-chitosan conjugate carrier for treatment of NRAS-mutant-harbouring lung carcinoma.

Carbohydrate polymers·2026
Same author

<i>In silico</i> screening of UM-C162 and UM-4E as potential inhibitors of <i>Staphylococcus aureus</i> virulence factors.

Journal of biomolecular structure & dynamics·2026
Same author

Multifaceted anticancer mechanisms of tribenzyltin carboxylates: DNA minor groove targeting and inhibition of key enzymes in DNA replication, nucleotide synthesis, and redox homeostasis.

Naunyn-Schmiedeberg's archives of pharmacology·2025
Same author

Inhibitory effects of UM-C162 on Staphylococcus aureus alpha-toxin-induced toxicity.

FEMS microbiology letters·2025
Same author

Discovery and Structure-Activity Relationship Study of Pyrazolyl Indolenine Derivatives as Staphylococcus aureus Biofilm Inhibitors.

Archiv der Pharmazie·2025
Same author

Selective Stabilization of LRRK2 Kinase Conformations Reveals Distinct Modes of Action for Type I and II Inhibitors.

Cell biochemistry and biophysics·2025
Same journal

Probing Cinnamamides and Benzamides as Anthelmintics: Discovery of Potent Drug-Like Agents Against Angiostrongylus cantonensis.

Chemical biology & drug design·2026
Same journal

Perfluorooctane Sulfonate Exposure and Peripheral Artery Disease: Mendelian Randomization and Integrative Multi-Omics Systems Toxicology With Machine Learning.

Chemical biology & drug design·2026
Same journal

In Silico Drug Repositioning Identifies SYK Kinase Inhibitors as Potential Neuroprotective Agents for Ataxia-Telangiectasia.

Chemical biology & drug design·2026
Same journal

Synthesis of Peptide Analogs From Fish Venom With Potential Antimicrobial and Anticancer Activity.

Chemical biology & drug design·2026
Same journal

Design, Optimization and Mechanistic Insights Into Terpenoid-Derived Inhibitors of Protein Kinase C Iota.

Chemical biology & drug design·2026
Same journal

Design, Synthesis, and In Vitro Biological Assessment of Novel Benzimidazole and Hydrazone Derivatives Against Pancreatic Cancer and Melanoma Cells.

Chemical biology & drug design·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.7K

Sofosbuvir as treatment against dengue?

Chye Sheng Gan1, See Khai Lim2, Chin Fei Chee1,3

  • 1Drug Design and Development Research Group, University of Malaya, Kuala Lumpur, Malaysia.

Chemical Biology & Drug Design
|August 24, 2017
PubMed
Summary
This summary is machine-generated.

Sofosbuvir, an anti-HCV drug, shows potential as a dengue treatment. Its active form inhibits dengue viral polymerase, demonstrating significant in vitro antiviral activity and suggesting a new therapeutic avenue for dengue infection.

Keywords:
GS-461203antiviraldenguenucleoside inhibitorsofosbuvir

More Related Videos

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
10:16

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

4.2K

Related Experiment Videos

Last Updated: Feb 24, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.7K
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
10:16

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

4.2K

Area of Science:

  • Virology
  • Drug Discovery
  • Biochemistry

Background:

  • Dengvaxia is the only licensed dengue vaccine but lacks WHO prequalification.
  • A universal dengue treatment is needed.
  • Nucleoside inhibitors targeting dengue viral polymerase are under investigation.

Purpose of the Study:

  • To evaluate the in silico and in vitro efficacy of sofosbuvir, an anti-HCV prodrug, against dengue virus.
  • To explore sofosbuvir's potential as a dengue polymerase inhibitor.

Main Methods:

  • In silico analysis of sofosbuvir's active metabolite (GS-461203) binding to dengue viral polymerase.
  • In vitro experiments to assess viral inhibition by sofosbuvir.
  • Investigation of the role of liver enzymes in sofosbuvir activation.

Main Results:

  • GS-461203 was predicted to bind to the dengue viral polymerase catalytic motif with high affinity (-6.9 kcal/mol).
  • Sofosbuvir exhibited potent in vitro viral inhibition with an EC90 of 0.4 μm.
  • Liver enzymes are required for sofosbuvir activation to its active metabolite.

Conclusions:

  • Sofosbuvir demonstrates significant potential as an antiviral agent against dengue.
  • The active metabolite of sofosbuvir effectively inhibits dengue viral polymerase.
  • Further studies are warranted to explore sofosbuvir's efficacy and potential as a dengue treatment in humans.