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Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS
Published on: January 14, 2013
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Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting
Tripp Leavitt1,2, Michael S Hu1,3,4, Michael T Longaker5,6
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2017
Summary
This study details a method for isolating live fibroblasts from mouse skin using fluorescence-activated cell sorting (FACS). This technique enables direct downstream analysis without altering cell characteristics through cell culture.
Area of Science:
- Cell Biology
- Immunology
- Biotechnology
Background:
- Flow cytometry is essential for real-time cellular phenotype characterization.
- Fluorescence-activated cell sorting (FACS) allows isolation of live cells for further experiments.
Purpose of the Study:
- To describe a method for isolating live fibroblasts from mouse dermal tissue using FACS.
- To provide an alternative to traditional cell culture methods that can alter cell characteristics.
Main Methods:
- Utilized fluorescence-activated cell sorting (FACS) for cell isolation.
- Targeted fibroblasts from the dermis of mouse skin samples.
Main Results:
- Successfully isolated live fibroblasts from mouse skin.
- Demonstrated a method that bypasses the need for ex vivo cell culture expansion.
Conclusions:
- FACS provides an effective means for isolating live fibroblasts from skin dermis.
- This method preserves cellular phenotype and function by avoiding ex vivo culture.
