Related Experiment Video
Updated: Aug 11, 2026
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North
X Li1, A Galliher-Beckley1, L Wang1
1Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Introduction:
Chinese HP-PRRSV characterized by high morbidity and mortality of all ages of pigs emerged since 2006 in China. The immune response of HP-PRRSV was never compared with conventional low pathogenic PRRSV strain.
Objective:
In this study, we compared the immune responses elicited by a Chinese HP-PRRSV strain HV and a North American RRSV strain NADC20 infections.
Result:
Pigs infected with NADC-20 showed significantly higher Ab titers than HV-PRRSV infected pigs at 9 DPI. Infection with HV-PRRSV induced a significantly higher levels of TNF-α and IL-10 in both sera and lung tissues and higher IFN-α and IFN-γ in the serum. Flow cytometry analysis showed that HV-PRRSV infected pigs generated significantly higher frequencies of NK cells in the peripheral blood and Th/memory, CTLs, and T-reg cells in the lung as compared with NADC-20 infected pigs.
Conclusion:
This study demonstrates that different immunity profiles were elicited by HV-PRRSV and NADC-20, and these differences may contribute to the distinct pathogenesis of HV-PRRSV and NADC-20.
More Related Videos
07:15Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Published on: November 22, 2021
06:57In Vitro Culture for H5N1-Specific Duck T Cells and Detection of Immune Responses Using Intracellular Cytokine Staining Method
Published on: May 30, 2025
