Multivariate Curve Resolution for Signal Isolation from Fast-Scan Cyclic Voltammetric Data

The use of multivariate analysis techniques, such as principal component analysis-inverse least-squares (PCA-ILS), has become standard for signal isolation from in vivo fast-scan cyclic voltammetric (FSCV) data due to its superior noise removal and interferent-detection capabilities. However, the requirement of collecting separate training data for PCA-ILS model construction increases experimental complexity and, as such, has been the source of recent controversy. Here, we explore an alternative method, multivariate curve resolution-alternating least-squares (MCR-ALS), to circumvent this issue while retaining the advantages of multivariate analysis. As compared to PCA-ILS, which relies on explicit user definition of component number and profiles, MCR-ALS relies on the unique temporal signatures of individual chemical components for analyte-profile determination. However, due to increased model freedom, proper deployment of MCR-ALS requires careful consideration of the model parameters and the imposition of constraints on possible model solutions. As such, approaches to achieve meaningful MCR-ALS models are characterized. It is shown, through use of previously reported techniques, that MCR-ALS can produce similar results to PCA-ILS and may serve as a useful supplement or replacement to PCA-ILS for signal isolation from FSCV data.