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Published on: December 10, 2011
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A facile and high sensitive micro fluorimeter based on light emitting diode and photodiode
Xuhui Geng1, Yan Gao1, Chunbo Feng1
1Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Talanta
|August 27, 2017
Summary
A new micro fluorimeter offers high sensitivity for detecting substances like fluorescein isothiocyanate (FITC) and chlorophyll. This portable device also achieves a low limit of detection for aflatoxin B1, crucial for fungimycin analysis.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Instrument Development
Background:
- Developing sensitive and efficient fluorimeters is crucial for various analytical applications.
- Existing fluorimeter designs can be complex and expensive, limiting their accessibility.
- There is a need for portable and cost-effective fluorescence detection systems.
Purpose of the Study:
- To develop a facile and highly sensitive micro fluorimeter.
- To optimize optical and electronic parameters for enhanced signal-to-noise ratio (SNR).
- To create a portable fluorimeter for specific applications like fungimycin detection.
Main Methods:
- Utilized a light-emitting diode (LED) as the light source and a photodiode (PD) as the detector.
- Optimized LED selection for complete cuvette irradiation and minimal divergence.
- Incorporated a reflector to enhance SNR and investigated PD amplifier circuits for sensitivity and stability.
Main Results:
- Achieved a 2.5-fold SNR enhancement with an optimized high-power LED.
- Doubled SNR by using a reflector.
- Established limits of detection (LOD) of 10pM (0.004 ppb) for fluorescein isothiocyanate (FITC) and 0.05 ppb for chlorophyll.
- Developed a portable version with a UV LED, achieving an LOD of 0.1 ppb for aflatoxin B1.
Conclusions:
- The developed micro fluorimeter demonstrates high sensitivity and improved SNR through optimized optical and electronic components.
- The portable version is effective for detecting specific contaminants like aflatoxin B1, indicating potential for fungimycin analysis.
- This facile and sensitive instrument offers a promising platform for various fluorescence-based detection applications.
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