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Plasmid
|August 27, 2017
PubMed
Summary

Interactions between three conjugative plasmids in bacteria show predictable transfer rate changes. If one plasmid hinders another, a third plasmid often has a similar effect, suggesting limited impact from the third plasmid

Keywords:
Conjugative plasmidsEscherichia coliFR1R124R16aR1drd19R388R477-1R57bR6KR702RN3RP4Transfer rate

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Area of Science:

  • Bacterial genetics
  • Molecular biology
  • Microbiology

Background:

  • Conjugative plasmids can interact within bacterial cells, altering their transfer rates.
  • Previous studies documented interactions between two conjugative plasmids, showing either decreased or increased transfer rates.

Purpose of the Study:

  • To investigate the interactions among three distinct conjugative plasmids within the same bacterial cell.
  • To determine how interactions between two plasmids influence the transfer rate of a third plasmid.

Main Methods:

  • Analyzing the transfer rates of conjugative plasmids in bacterial cells containing multiple plasmids.
  • Quantifying plasmid interactions across various combinations of three distinct conjugative plasmids.

Main Results:

  • Plasmids interact in a significant majority (59/84) of combinations.
  • Interactions are largely consistent: if plasmid A decreases plasmid B's transfer rate, plasmid C often does too.
  • The effect of a third plasmid is generally independent of its identity, though it can quantitatively modulate existing interactions.

Conclusions:

  • Conjugative plasmid interactions in multi-plasmid bacterial systems exhibit predictable patterns.
  • The presence of a third plasmid typically reinforces the interaction dynamics established by two plasmids.
  • A bias towards negative interactions (decreased transfer rates) has positive implications for controlling the spread of antibiotic resistance and virulence factors.