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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Feb 24, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.

Roxanne E Diaz¹, Aurore Sanchez¹, Véronique Anton Le Berre²

¹Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Centre National de la Recherche Scientifique (CNRS), Université de Toulouse, UPS, F-31000, Toulouse, France.

Methods in Molecular Biology (Clifton, N.J.)

|August 27, 2017

Summary

Chromatin immunoprecipitation sequencing (ChIP-seq) maps DNA-binding proteins in bacteria. This study details methods for obtaining high-quality DNA for high-resolution ChIP-seq data and bacterial nucleoid studies.

Keywords:

Affinity-purified antibody Bacterial nucleoid Chromatin immunoprecipitation DNA fragmentation DNA sonication DNA target Genome-wide maps Next-generation sequencing

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Last Updated: Feb 24, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

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Published on: December 10, 2014

Area of Science:

Microbiology
Molecular Biology
Genomics

Background:

Chromatin immunoprecipitation (ChIP) combined with next-generation sequencing (NGS) is a key technique for analyzing DNA-protein interactions.
Understanding nucleoprotein components is crucial for bacterial nucleoid organization and cellular processes.

Purpose of the Study:

To outline the essential steps for generating high-quality immunoprecipitated DNA.
To enable high-resolution ChIP-sequencing (ChIP-seq) data for bacterial genomics.

Main Methods:

Detailed protocol for Chromatin immunoprecipitation (ChIP).
DNA fragmentation and purification for library construction.
Optimization of immunoprecipitated DNA for NGS.

Main Results:

Achieved short, specific, and high-quality immunoprecipitated DNA fragments.
Established a robust workflow for ChIP-seq library preparation.
Facilitated high-resolution genome-wide mapping of DNA-binding proteins.

Conclusions:

The described methodology enhances the quality and resolution of ChIP-seq data in bacteria.
This approach is vital for detailed studies of bacterial nucleoid structure and function.