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Updated: Aug 11, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
A Chromosome Co-Entrapment Assay to Study Topological Protein-DNA Interactions
Larissa Wilhelm1, Stephan Gruber2,3
1Research Group 'Chromosome Organization and Dynamics', Max Planck Institute of Biochemistry, Martinsried, Germany.
Abstract:
Chromosome organization, DNA replication, and transcription are only some of the processes relying on dynamic and highly regulated protein-DNA interactions. Here, we describe a biochemical assay to study the molecular details of associations between ring-shaped protein complexes and chromosomes in the context of living cells. Any protein complex embracing chromosomal DNA can be enriched by this method, allowing for the underlying loading mechanisms to be investigated.
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